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rfp expressing u87 cells  (ATCC)


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    ATCC rfp expressing u87 cells
    Rfp Expressing U87 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 10908 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rfp expressing u87 cells/product/ATCC
    Average 99 stars, based on 10908 article reviews
    rfp expressing u87 cells - by Bioz Stars, 2026-03
    99/100 stars

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    Co-culture of primary glial cultures from irradiated mouse brains <t>with</t> <t>GBM</t> cells. ( A ) Microphotograph of the co-culture of <t>U87</t> GBM cells with glial cells from the control or irradiated brain tissues. Magnification ×200, scale bar 500 µm. ( B ) Quantitative analysis of a number of U87 cells per 0.5 × 0.5 cm square (Zeiss LSM710 tile-scan + CellProfiler 4.1.3). * p < 0.05. ( C ) Immunofluorescence analysis of PG core proteins (decorin, brevican) and GAG content (CS, HS) in primary glial cells co-cultured with U87 GBM cells or triple-irradiated or both. Visualization of the studied antigens with Alexa Fluor 488 secondary antibodies (green), <t>U87-RFP</t> cells (red) and DAPI (blue). Magnification ×400,scale bar 100 µm. ( D ). Quantitative analysis of the PG core proteins and GAG content in the glial cultures from control and irradiated mouse brain tissues (CellProfiler4.1.3. software). ANOVA, * p < 0.05, ** p < 0.01, *** p < 0.001.
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    Co-culture of primary glial cultures from irradiated mouse brains <t>with</t> <t>GBM</t> cells. ( A ) Microphotograph of the co-culture of <t>U87</t> GBM cells with glial cells from the control or irradiated brain tissues. Magnification ×200, scale bar 500 µm. ( B ) Quantitative analysis of a number of U87 cells per 0.5 × 0.5 cm square (Zeiss LSM710 tile-scan + CellProfiler 4.1.3). * p < 0.05. ( C ) Immunofluorescence analysis of PG core proteins (decorin, brevican) and GAG content (CS, HS) in primary glial cells co-cultured with U87 GBM cells or triple-irradiated or both. Visualization of the studied antigens with Alexa Fluor 488 secondary antibodies (green), <t>U87-RFP</t> cells (red) and DAPI (blue). Magnification ×400,scale bar 100 µm. ( D ). Quantitative analysis of the PG core proteins and GAG content in the glial cultures from control and irradiated mouse brain tissues (CellProfiler4.1.3. software). ANOVA, * p < 0.05, ** p < 0.01, *** p < 0.001.
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    Co-culture of primary glial cultures from irradiated mouse brains with GBM cells. ( A ) Microphotograph of the co-culture of U87 GBM cells with glial cells from the control or irradiated brain tissues. Magnification ×200, scale bar 500 µm. ( B ) Quantitative analysis of a number of U87 cells per 0.5 × 0.5 cm square (Zeiss LSM710 tile-scan + CellProfiler 4.1.3). * p < 0.05. ( C ) Immunofluorescence analysis of PG core proteins (decorin, brevican) and GAG content (CS, HS) in primary glial cells co-cultured with U87 GBM cells or triple-irradiated or both. Visualization of the studied antigens with Alexa Fluor 488 secondary antibodies (green), U87-RFP cells (red) and DAPI (blue). Magnification ×400,scale bar 100 µm. ( D ). Quantitative analysis of the PG core proteins and GAG content in the glial cultures from control and irradiated mouse brain tissues (CellProfiler4.1.3. software). ANOVA, * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: International Journal of Molecular Sciences

    Article Title: Multiple Irradiation Affects Cellular and Extracellular Components of the Mouse Brain Tissue and Adhesion and Proliferation of Glioblastoma Cells in Experimental System In Vivo

    doi: 10.3390/ijms222413350

    Figure Lengend Snippet: Co-culture of primary glial cultures from irradiated mouse brains with GBM cells. ( A ) Microphotograph of the co-culture of U87 GBM cells with glial cells from the control or irradiated brain tissues. Magnification ×200, scale bar 500 µm. ( B ) Quantitative analysis of a number of U87 cells per 0.5 × 0.5 cm square (Zeiss LSM710 tile-scan + CellProfiler 4.1.3). * p < 0.05. ( C ) Immunofluorescence analysis of PG core proteins (decorin, brevican) and GAG content (CS, HS) in primary glial cells co-cultured with U87 GBM cells or triple-irradiated or both. Visualization of the studied antigens with Alexa Fluor 488 secondary antibodies (green), U87-RFP cells (red) and DAPI (blue). Magnification ×400,scale bar 100 µm. ( D ). Quantitative analysis of the PG core proteins and GAG content in the glial cultures from control and irradiated mouse brain tissues (CellProfiler4.1.3. software). ANOVA, * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: For co-culture experiments, human GBM U87 cells stably expressing RFP (U87-RFP cells) (AntiCancer Inc., San Diego, CA, USA) were used.

    Techniques: Co-Culture Assay, Irradiation, Control, Immunofluorescence, Cell Culture, Software

    Co-culture of organotypic brain tissue slices ex vivo with GBM cells. ( A ) Effects of multiple irradiation on PGs’ expression in brain tissue. Real-time RT–PCR analysis, intensity of the amplified DNA fragments normalized to that of Gapdh , bars represent the mean ± SD from triplicate experiments, Student t-test (OriginPro 8.5). ( B ) Overall transcriptional activity of PG core protein-coding genes in brain tissue from control and irradiated mouse brains. The stacked columns compare the contribution of each value to a total across categories. ( C ) Confocal microscopy of U87-RFP cells seeded on the control and irradiated brain slices. Cells nuclei are stained with DAPI. Scale bar 500 μm. ( D ) Quantitative analysis of the U87-RFP cells on the control and irradiated brain tissues. ANOVA and post-hoc Fisher’s LSD test, * p < 0.05, ** p < 0.01.

    Journal: International Journal of Molecular Sciences

    Article Title: Multiple Irradiation Affects Cellular and Extracellular Components of the Mouse Brain Tissue and Adhesion and Proliferation of Glioblastoma Cells in Experimental System In Vivo

    doi: 10.3390/ijms222413350

    Figure Lengend Snippet: Co-culture of organotypic brain tissue slices ex vivo with GBM cells. ( A ) Effects of multiple irradiation on PGs’ expression in brain tissue. Real-time RT–PCR analysis, intensity of the amplified DNA fragments normalized to that of Gapdh , bars represent the mean ± SD from triplicate experiments, Student t-test (OriginPro 8.5). ( B ) Overall transcriptional activity of PG core protein-coding genes in brain tissue from control and irradiated mouse brains. The stacked columns compare the contribution of each value to a total across categories. ( C ) Confocal microscopy of U87-RFP cells seeded on the control and irradiated brain slices. Cells nuclei are stained with DAPI. Scale bar 500 μm. ( D ) Quantitative analysis of the U87-RFP cells on the control and irradiated brain tissues. ANOVA and post-hoc Fisher’s LSD test, * p < 0.05, ** p < 0.01.

    Article Snippet: For co-culture experiments, human GBM U87 cells stably expressing RFP (U87-RFP cells) (AntiCancer Inc., San Diego, CA, USA) were used.

    Techniques: Co-Culture Assay, Ex Vivo, Irradiation, Expressing, Quantitative RT-PCR, Amplification, Activity Assay, Control, Confocal Microscopy, Staining